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Effect on cell viability was assessed 72 h after transfection with GSTO1 siRNA or siSCRAM by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and normalized to mock-transfected control (transfection reagent)
I have really hard to get veins and they are fantastic about avoiding digging around to get one
33 This article updates previous articles on this topic by Langan and Zawistoski, 4 and by Oh and Brown
Absorbance (418 nm) was assessed using a plate reader set at 37 C with a total reading time of 4560 min and 15 min intervals between reads
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