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Bacterial growth conditions for flow cytometry and qPCR Bacteria were grown for 18 h at 37 C with shaking in 2 ml of a minimal defined medium [21 mM K 2 HPO 4 , 11 mM KH 2 PO 4 , 3.8 mM (NH 4 ) 2 SO 4 , ~ 3.8 mM KOH, 25 mM glycerol, 1 mM MgCl 2 , 10 M FeCl 3 , 1 mM NaCl, 1 MEM amino acid solution (Gibco), 1 MEM nonessential amino acid solution (Gibco), pH 7.4] 8
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Vallespinos M, Fernandez D, Rodriguez L, Alvaro-Blanco J, Baena E, Ortiz M, et al
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