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The messenger RNA (mRNA) expression levels of the target genes were determined by the 2 Ct method, using the following formula: [ Ct = (Ct gene of interest Ct actin ) treated (Ct gene of interest - Ct actin ) untreated ] (Li et al., 2015)
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The infected mice exhibited a higher increase in interleukin (IL) IL-5, IL-4, and IL-2 than IL-12 and interferon- (IFN-), suggesting an important alteration in cytokine profile from Th1 to Th2 (Figure 2) [38]
59 DesmetA.-S.CirilloC.TackJ.VandenbergheW.Vanden BergheP
Proteome-wide analysis of USP14 substrates revealed its role in hepatosteatosis via stabilization of FASN
