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GLP-1R co-localisation with clathrin-coated pits (CCPs) with using TIRF microscopy INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were transfected with clathrin light chain (CLC)-GFP and plated onto poly-L-lysine-coated glass coverslips
We found that the cre-dependent ablation of NTS Ppgfl early in development or in adult mice failed to alter energy balance, suggesting the importance of pathways independent of NTS GLP1 for the long-term control of food intake
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Brain Dis
The membrane was washed three times with TTBS for 5 min each and incubated with horseradish peroxidase conjugated goat anti-mouse IgG (Sigma) diluted (1:3000) in TTBS at 37C for 1 h
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