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[5] Sikiric P, et al

To analyze the glutaredoxin-dependent oxidation kinetics for recombinant roGFP2, we first confirmed the fluorescence spectra of reduced and oxidized wild-type roGFP2 (roGFP2 WT (SH) 2 and roGFP2 WT (S 2 ), respectively) using a stopped-flow spectrofluorometer (Fig