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Abstract The spectrophotometric/microplate reader assay method for glutathione (GSH) involves oxidation of GSH by the sulfhydryl reagent 5,5-dithio-bis(2-nitrobenzoic acid) (DTNB) to form the yellow derivative 5-thio-2-nitrobenzoic acid (TNB), measurable at 412 nm

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Subacute and chronic injuries predominantly feature demyelination of the nervous system and megaloblastic anemia, with up to 96% of patients experiencing neurological damage (Oussalah et al., 2019

The concentration is high enough to keep injection volumes reasonable while making dose calculations straightforward