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Troubleshooting: Cloudy or Undissolved Solution If, after the passive mixing phase and gentle swirling (Step 2.3), the solution remains cloudy, foamy, or contains undissolved particulate matter, one of the following issues has likely occurred [1]: Peptide Denaturation : The peptide structure was damaged by injecting the water too forcefully

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If those parameters are relevant to your work, note their absence before use