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For a 2.5 mg target dose: Volume to Draw = Desired DoseConcentration = 2.5 mg10 mg/mL = 0.25 mL Convert to syringe units

BBB, composed of brain endothelial cells, astrocytes, pericytes, neurons, supporting structures (microglia, oligodendrocytes), and extracellular matrix (ECM), acts as a selective barrier regulating the exchange of substances between the bloodstream and brain parenchyma while preventing the entry of harmful substances [10]

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