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The Nocturnal Window: The largest natural pulse of growth hormone occurs during the first few hours of deep, slow-wave sleep

Two symbols (**, ##, or $$) denote significant difference below 0.005, three symbols (***, ###, or $$$) denote significant below 0.0001

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Time-course studies track GH-axis markers at multiple intervals after a single administration to characterize onset and duration, which is particularly relevant when comparing the spray format against injectable delivery

Protein bands were visualized using enhanced chemiluminescence (ECL) and captured using a ChemiDoc MP imaging system (Bio-RAD, Shanghai, China)