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The assay buffer consisted of DMEM without phenol red (Gibco #11880-028) supplemented with 10 mM HEPES (Gibco #15630-056), 1X GlutaMAX (Gibco #35020-038), and 1% (w/v) ovalbumin (Sigma A5503), 0.1% (v/v) Pluronic F-68 (Gibco, #24040-032)

HGH plays a key role in: Linear growth (especially in childhood) Muscle and bone development Fat metabolism Wound healing and tissue repair Immune function Cognitive health and mood regulation Once released, HGH stimulates the liver and other tissues to produce IGF-1 (Insulin-like Growth Factor 1) the primary mediator of most growth-related effects in adults

Melatonin also regulates matrix metalloproteinases (MMPs), enzymes known to compromise BBB integrity [195]

Gut healing is most effective when combined with supportive lifestyle habits, including proper nutrition and stress management

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