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J.Barba-EspnG.Daz-VivancosP.RubioM.DicentaF.et al
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HEK293T cells were transfected with 1 g lentiCRISPRv2 containing the sgRNA insert, 0.67 g psPAX2 (Addgene, 12260) and 0.33 g pVSVg (Addgene, 8454) using 6 g polyethylenimine (Sigma-Aldrich, 919012) in Opti-MEM (Thermo Fisher, 31985062)
Proinflammatory cytokines compromise ECs, stimulate the release of ICAM, and enhance the adhesion and infiltration of immune cells
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