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GltT-defective mutants (NMB1965- and/or NMB1964-defective mutants, respectively) were significantly defective in the internalization into human umbilical vein endothelial cells (HBMEC) and the human lung carcinoma epithelial cell line A549
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The outputs from GD_SS1.1 and GD_SS1.2 were pooled and evolved using PANCE on the second stepping-stone, GD_SS2, a substrate containing five additional substitutions from gasdermin D (Fig
Progress photos are the raw visual log
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