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What is measured in these rats and mice (and occasionally in small groups of human volunteers) is often not a hard endpoint like muscle strength or weight loss, but surrogate biomarkers: molecules in the blood that have an indirect link to a hard endpoint
A claim may be denied when a patient submits a D50.9 to a provider when there is documentation of blood loss
Very lean individuals should pinch the skin firmly before injection to create a fold of subcutaneous tissue
J Physiol 559:169186 Aoyama K, Watabe M, Nakaki T (2008) Regulation of neuronal glutathione synthesis
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