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Nevertheless, this guide has illuminated key areas where further TB-500 investigation may be warranted, providing researchers with a useful starting point for future studies
Storage: Store in a cool, dry environment away from direct light and moisture
DNA amplifications were performed in a Bio-Rad CFX-96 real-time thermocycler (Bio-Rad, USA), running a standard program (95C for 2 min, 40 cycles at 95C for 10 s, 60C for 10 s and 72C for 30 s, followed by a melting curve analysis to determine amplicon specificity using a temperature range from 65 to 95C with increments of 0.5C)
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Appetite scores decreased by an average of 26.5 points, while cravings for sweet and savory foods dropped by 2.7 and 2.3 points, respectively
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