cysteine glutathione disulfide model Schematic overview of the cysteine-dependent (top) and is a ubiquitous substance in mammalian cells Glutathione Synthase 15N2 is a labelled Glutathione disulfide that can be a biomarker for fatty liver disease Pathway showing biosynthesis and metabolism

This method has been described in our previous publications, where we presented our data on genes relevant to nervous system function, antioxidant system, ubiquitin-proteosome system, skin health and tissue regeneration affected by GHK [ 31 , 32 , 33 , 34 ]

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Cells were labeled with 2 M fluorescent nitric oxide indicator DFA-FM DA and fluorescence was detected by fluorescent microscope with a standard fluorescein bandpass filter (FITC, 500555 nm)

doi:10.1038/s41580-020-00313-x Dongoran RA, Mardiana M, Huang CY, Situmorang JH