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We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )
Copper chelation was required: the GHK tripeptide alone (without copper) was markedly less active
FDA and similar regulators worldwide have not approved GHK-Cu as a drug or supplement, meaning it cannot be sold for human or medical application
Semax: from ACTH4-10 to the neurotrophic tool Origin and structure Semax is a synthetic heptapeptide developed in the 1980s at the Institute of Molecular Biology in Moscow
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