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The COPD-C model was achieved by administering 80 U/kg of Porcine Pancreatic Elastase (PPE, Elastin Products Company, Owensville, MI, USA) in 50 L of Phosphate Buffered Saline (PBS) through intratracheal (i.t.) instillation using a microsprayer aerosolizer from Penn Century Inc., located in Wyndmoor, PA, USA on day 0

This is the primary clinical rationale for IV administration when therapeutic-level replenishment is the goal

Anti-Ageing Effects: Decreases oxidative stress, helping to slow the ageing process

Approximately 5% of the inhaled O 2 is converted to reactive oxygen molecules with an unpaired number of electrons such as hydroxyl (OH), superoxide anion (O 2 -), hydrogen peroxide (H 2 O 2 ), oxygen singlet (O 2 ), hypochlorite (OCI-), nitric oxide (NO), and peroxynitrite (ONOO-) radicals

It is best employed as adjunctive therapy to medical management & works best when combined with lasers & or chemical peels