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However, the precise enzymatic machinery responsible for Kla deposition and removal remains incompletely characterized and the relative contributions of enzymatic vs

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To generate stable cell lines, MYTHO cDNA was cloned into the pDONR223 vector (Addgene) by using the BP Clonase Reaction Kit (Thermo Fisher Scientific) and further recombined into the lentiviral GATEWAY destination vector pLenti-UBC-gate-3xHA-pGK-PUR (107393, AddGene)

Protein quantification was performed using Pierce BCA protein assay kit (Thermo Scientific, 23225)