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Briefly, 1.5 g plasmids DNA and 3 L of 1 mg/ml Polyethylenimine (PEI, 408,727-sigma) transfection reagent were diluted in 250 L of FBS-free DMEM (Invitrogen) separately
Research-grade L-carnitine requires 99% chiral purity (L-form only) verified by chiral HPLC, with absence of the biologically inactive D-carnitine isomer
& Yoo, S
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The bioenergetic and antioxidant status of neurons is controlled by continuous degradation of a key glycolytic enzyme by APC/C-Cdh1
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