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Amino acids from protein serve as building blocks for neurotransmitters
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Then the wells were washed twice with wash buffer, and diluted streptavidin-RPE reporter reagent (diluted following manufacturers instructions) was added to all wells
Features detected in less than 50% of the QC samples or 80% of the biological samples were excluded the remaining peaks with missing values were imputed with the k-nearest neighbor algorithm to further improve the data quality
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