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The perfused pancreas was digested at 37 C for 13 min with a short vortex every 3 min, and the islets were processed with gradient centrifugation with Pancoll human solution (PAN-Biotech) and then handpicked under a stereoscopic microscope
Deficiencies can occur due to low feed intakes or more commonly as a result of vitamin breakdown by oxidation during the processing and storage of grains and hays
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The absorbance values of the wells were measured at 450 nm using a microplate reader
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