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SEC was performed by loading the protein onto Superose 6 Increase 10/300GL (GE Healthcare) column with running buffer containing 20 mM HEPES, pH 7.4, 100 mM NaCl, 10 mM MgCl 2 , 100 M TCEP, 5 M tirzepatide, 0.00075% (w/v) LMNG, 0.00025% (w/v) GDN, 0.0002% (w/v) CHS and 0.00025% digitonin (Anatrace)
Burnham, MD is a board-certified orthopedic surgeon and Director of Sports Medicine at Ochsner-Andrews Sports Medicine Institute in Baton Rouge, Louisiana
The GT activity was calculated considering the CDNB extinction coefficient (9.6 mM 1 cm 1 )
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