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Benefits will vary based on ones' lifestyle therefore the best guage for frequency will be how you feel.

Garza A, et al

Refrigeration and the preservative in bacteriostatic water extend usable life to weeks for many peptides

[6] [3] [10] A mild deficiency may not cause any discernible symptoms, but moderate deficiency can cause various symptoms, such as feeling tired, lightheadedness, headaches, cold intolerance, pale skin, sore tongue, upset stomach, loss of appetite, hair and skin color changes, numbness or tingling (pins and needles) of the fingers and toes, and tinnitus, and vision, cognitive or psychological problems

Cycles may range from approximately 21 to 45 days