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Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts

A 2022 study in Diabetes, Obesity and Metabolism showed that NNMTi treatment combined with reduced-calorie diet "promoted dramatic whole-body adiposity and weight loss in diet-induced obese mice, rapidly normalizing" weight parameters

### Step 6: Let It Dissolve Set the vial down and wait 1-2 minutes

While three forms of cystinosis have been described, the disorder displays a continuum of clinical severity inversely related to the amount of residual cystine-transporting activity