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The (His) 6 -tag was removed by overnight thrombin cleavage, followed by polishing with size-exclusion chromatography (SEC) using a Superdex 75 HiLoad 16/600 column (Cytiva, MA, USA) equilibrated in 20 mM Tris-HCl pH 8.0, and 150 mM NaCl
We cannot verify with certainty that patients continuously took the medications throughout the study period, Dr
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Supplements Unlike lipoic acid in foods, lipoic acid in supplements is not bound to protein
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