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A previous study demonstrated that bicistronic expression of RFP and GFP was at a comparable level as their monocistronic expression using a 2 A peptide sequence from the equine rhinitis B virus to link the two gene sequences [48]

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After that, the mixture was transferred into the column and pre-washed with wash buffer (PBS, contained 0, 10, or 30 mmol/L imidazole, pH = 7.6), followed by elution buffer (PBS, contained 300 mmol/L imidazole, pH = 7.6)

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