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Cell culture and transfection Gastric cancer cell lines SNU638 (KCLB No.00638), SNU216 (KCLB No.00216), and SNU668 (KCLB No.00668) cells were obtained from KCLB (Korean Cell Line Bank), and HEK293T (CRL-3216) cells were obtained from ATCC (American Type Culture Collection)
Quantitative analysis was performed using ImageJ software by counting the number of Nissl-positive neurons in the hippocampal CA1, CA3, and dentate gyrus (DG) regions
A complete kit includes: A sealed vial or glass ampoule of B12 (cyanocobalamin or methylcobalamin) A 3 mL syringe A drawing needle (typically 18-21 gauge) An injecting needle: 22-25 gauge, 1 to 1.5 inch for intramuscular (IM)
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