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Highly purified water (typically water for injection, which has undergone multiple distillation or reverse osmosis) is mixed with a precisely measured amount of benzyl alcohol so that the resulting concentration is exactly 0.9% (weight/volume)

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Reconstitution: The Basics Choosing Your Dilution The amount of bacteriostatic water you add determines the concentration of your solution: 250mg vial options: 2.5mL water = 100mg/mL (1mL = 100mg) 5mL water = 50mg/mL (1mL = 50mg) 10mL water = 25mg/mL (1mL = 25mg) Recommendation: For most research purposes, a 5mL dilution (50mg/mL) provides a good balance of manageable injection volumes and precise dosing