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Differential induction of glutathione S-transferase in rat aorta versus liver [PMID: 8204108] Laboratory literature summary: Differential induction of glutathione S-transferase in rat aorta versus liver

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Streptavidin pulldown HeLa WT cells were seeded in a 10 cm dish and harvested after 48 h in PBS, lysed in 300 l RIPA buffer per sample (50 mM Tris-HCl pH 7.5, 0.1 % SDS, 0.5 % Sodium deoxycholate, 1mM EDTA, 150 mM NaCl, addition of protease inhibitors freshly) and incubated for 10 min on ice

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