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During molecular dynamic simulations with GLP-1 bound, the N-terminal helix was oriented vertically (black and blue arrows), whereas in the apo-form the ECD N-terminal helix was more dynamic and experienced both open and closed conformations (this is analogous to the suggested ECD dynamics for the glucagon receptor)
Following dissociation, islets cells were washed 3 times with PBS containing 0.01% BSA, followed by centrifugation at 352 g for 5 minutes, and then resuspended in PBS containing 0.01% BSA at a concentration of approximately 250,000 cells/mL, which was used for downstream DROP-Seq and DART-Seq experiments
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Briefly, 500 ng of genomic DNA per sample was bisulfite-converted (Zymo EZ DNA Methylation kit) and hybridized to the EPIC array, with arrays scanned on an iScan instrument to produce raw intensity data
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