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Methods Cell culture, expression constructs, and transfection MIN-6 (purchased from ATCC) cells and GLUTag cells (obtained from Takashi Tsuboi, the university of Tokyo, Japan) were cultured in DMEM with 10% (v/v) heat-inactivated fetal bovine serum

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Consequently, elucidating the underlying pathophysiological mechanisms of RIRI and developing targeted therapeutic strategies hold substantial clinical relevance

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75 For example, in prostate cancer, loss of the histone methyltransferase KMT2C represses ASPP2 via impaired enhancer activity