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Figure 1 Culturing in Ringer Solution at Low Hematocrit Increases the Baseline Levels of PS Exposure and Hemolysis in Erythrocytes To test the influence of specific culture conditions on baseline levels of eryptosis, and in the absence of any drug, nave erythrocytes were incubated in either Ringer solution (at 0.4 or 4% hematocrit) or in cRPMI at 4% hematocrit ( Figures 2A, B )

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By 48 hours, the DE markers forkhead box protein ( FOXA2 ), GSC, SOX17 , hematopoietically expressed homeobox ( HHEX ), and cerberus ( CER1 ) were robustly expressed

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