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Your commitment to quality means knowing when to discard a compromised sample

The assays were carried out in a total volume of 100 L per well in a black 96-well plate

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Lactate Dehydrogenase (LDH) Assay The relative amounts of LDH released into the media (conducted in triplicates for each group) were determined using CytoTox-ONE Homogeneous Membrane Integrity Assay kit Cat.#G7892 (Promega, USA) according to the manufacturers instructions