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Retinal primary mixed cell culture P0-P4 neonates of Long Evans were euthanized by direct decapitation, retinas dissected and washed using DMEM medium before the retinas were digested with a 2.5% trypsin solution for 10 min at 37 C (Thermo Fisher, #15090046)

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The two sclerosants were identical in composition

For example, 70% of substrates with a log 2 (fold enrichment) greater than 2.0 generated a luminescent signal (Extended Data Fig