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After 24 h, the HFF-1 cells in the well plates were imaged using phase contrast microscopy (Zeiss, Axio, Germany) for growth appearance, adherence and confluence

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Then, L-theanine alone or in combination with theobromine could effectively inhibit tumor production in male Wistar rats with colon cancer induced by dimethylhydrazine, and the mechanism of action was related to downregulating the Akt/mTOR (mammalian target of rapamycin) and JAK2/STAT3 pathways and increasing the mRNA and protein expression of tumor suppressor Smad2 (73)