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berghei transfections were accomplished by electroporation of purified schizonts using the Amaxa Nucleofector Technology (Lonza) as described previously ( pbgst - ko 1A and 1B , containing the tgdhfr/ts selectable marker, were selected with pyrimethamine as previously described ( pbgst - ko 2A and 2B , which contains the hdhfr selectable marker, were selected with WR99210 ( pbgst-ko 2A and 2B were done in parallel with a previously described plasmid targeting the pbmrp as a transfection control ( Integration of knockout plasmids was verified by Southern analysis of chromosomes separated by CHEF or FIGE
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