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RT-qPCR assay To investigate the gene expression levels of Pelo, Hbs1, E3, and Hsp70 in insect vectors, the total RNAs of 50 intact insects infected with RGDV or treated with dsRNAs were extracted using TRIzol reagent (Invitrogen) following the manufacturers instructions
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Mechanism gaps Despite four decades of research, DSIP precise mechanism of action remains unclear
Martchenko A, Oh RH, Wheeler SE, Gurges P, Chalmers JA, Brubaker PL
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