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Funding This paper was supported by San Matteo Hospital Foundation, Internal Medicine research fundings, PRIN2017. Publishers note All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers
0.25mg dose, would require only 0.05ml, you would need very accurate measuring
However, this method has limitations in both sensitivity and specificity such as instability of the DTNB reagent and interference from free thiols like glutathione 29
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A thorough medical evaluation, including blood work to check IGF-1 levels and overall hormone status, is the safest first step before starting therapy
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