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Then cDNA was synthesized using SuperScript TM IV VILO TM Master Mix (Invitrogen, Waltham, MA, United States) and diluted 101 by DNase/RNase-Free Distilled Water (Invitrogen)
The faint blue color of the reconstituted solution is normal
When this muscle becomes inflamed or overused, this muscle can impinge on or irritate the sciatic nerve deep in the buttock, causing sciatica
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