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We are constantly exposed to environmental factors like pollution, UV rays, and processed foods that increase the burden of free radicals

Mitochondrial glutathione oxidation leads to cell death In this study, we have demonstrated that oxidation of the mitochondrial glutathione pool, driven almost exclusively by Prx1 activity, is an important determinant of cell death upon exposure to an acute H 2 O 2 stress (Fig 7E)

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For endothelial cells, which were cultured in a confocal dish, they were fixed with paraformaldehyde at room temperature for 15 min