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Importantly, decreased Y198- and Y250-nitration was visualized by re-probing the blots with 3A11C6 anti-nitroY198-HGF and 6B82C3 anti-nitroY250-HGF-specific mAbs, respectively (Figure 1b,c, first rows)

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once reconstituted, store refrigerated at 28C and use within the documented research window, or aliquot and freeze to avoid repeated freeze-thaw cycles

The compound's triple agonist mechanism may be considered sufficiently comprehensive that additional combinations aren't prioritized in the development pipeline

This effect translated into a reduction of claudin-5 protein with significant suppression at doses of 0.5 ng/ml rTGF 1 and greater (Figure 4b)