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Strengths, limitations, and recommendations This study has notable strengths, including a controlled four-group design enabling independent and combined evaluations of exercise and chromium supplementation, the use of a validated chronic treadmill protocol, and the simultaneous assessment of oxidative stress (MDA, AOPP) and antioxidant markers (CAT, GSH), providing a comprehensive redox profile
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An equal volume of dye Mastermix (1 M JC-1, 0.005% digitonin, 10 g ul 1 oligomycin, 5 mM -mercaptoethanol in DTEP buffer) was added, and after 10 min at room temperature, the cells were added on top of the peptide template at a concentration of 40,000 cells per well
coli cytoplasm (Derman et al., 1993)
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