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Relative quantification was performed by normalizing the sum of the AUC of all isotopologues for each metabolite to the sum of the AUC of all isotopologues of norvaline within each sample followed by normalization to the average protein quantification within each experimental group
This can help locate the hyperpigmentation to verify if it is on the sclera (the white of the eye) or the conjunctiva (the layer on the outer surface of the eye)
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