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ELISA assay After euthanizing the mice, the plasma samples collected from experimental animals blood were obtained by centrifugation at 3000 rpm for 15 min

What to watch for in results Declining albumin levels may indicate inadequate protein intake

Both approaches complement each other

Pyridoxine is also considered safe during lactation in normal amounts, although exceedingly high doses might transiently suppress prolactin levels and were historically used (in much larger doses than nutritional needs) to suppress lactation