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cDNA synthesis was performed with the PrimeScript RT Reagent Kit (Takara, RR047A, Japan)
Signs of low testosterone in men include: low sex drive (libido) decreased muscle mass erectile dysfunction lethargy/low energy increased body fat The most serious form of low-T is known as pathologic hypogonadism, and indicates a persistent reduction in testosterone levels caused by an irreversible condition
Acknowledgments We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript
(2013) 3:e001570
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