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A GST mutant in which four glutamine residues were replaced with asparagine (GST 4QN ) exhibited a glutathione binding affinity similar to that of wild-type GST and could be purified by glutathione affinity chromatography

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The small molecule drug discovery process begins with identifying a biological target and progresses through hit identification, hit-to-lead evolution, and lead optimisation before compounds enter preclinical in vivo and clinical testing

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