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That single fractionation study is the proof point researchers still cite more than fifty years later as the origin of the entire copper-peptide research field: a compound identified almost by accident in a liver-aging comparison went on to become one of the most studied metallopeptides in dermal and regenerative research

14 Current assays have limited capacity to distinguish among distinct resistance pathways, such as target mutations, bypass pathway activation, or phenotypic transitions

In severe cases, skin and underlying tissue can suffer necrosis (tissue death), requiring antibiotics or surgery to treat

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