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For HUVEC and HDMEC, plates were pre-coated with gelatin (Difco Laboratories, 0.1% for 1 h at 37 C)

However, there is a low risk of vitamin B12 toxicity because the body only utilizes what it needs and eliminates the excess

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GHK-Cu (glycyl-L-histidyl-L-lysine-copper) is a naturally occurring tripeptide - three amino acids bound to a copper ion

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