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HUVECs were incubated in an endothelial cell growth medium (PromoCell, Heidelberg, Germany) containing an endothelial cell growth supplement

As an emerging clinical model, PDOs hold great promise not only for elucidating resistance mechanisms but also for drug screening and guiding individualized therapeutic strategies

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Nicotinamide Adenine Dinucleotide (NAD) is a coenzyme found in all living cells

Corroborating these findings, trajectory analysis demonstrated distinct exploration patterns across groups (Fig