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After five washing steps with PBS-Tween, the secondary antibody (Alexa Fluor 555 anti-mouse, Life Technologies, 1:200) was added in 2% BSA/PBS-Tween together with Hoechst 33342 (1 g/ml) for 2 h

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Labs may order the test again if results come back as inconclusive

Another study showed that glutathione stores were increased with the use of a liposomal product

Nrf2, a pivotal transcription factor responsible for maintaining cellular metabolism and redox balance, governs various molecules associated with ferroptosis, including FT light and heavy chains (FTL/FTH1), the system Xc subunit, the catalytic and regulatory subunits of glutamic acid cysteine ligase (GCLC/GCLM), as well as GPX4 [64, 65]